University of Glasgow
Although blood culture remains a gold-standard to detect the presence of infectious agents and inform antibiotic treatment in infected patients, time to results (days) and false negative diagnostics for hard-to-culture pathogens can have devastating impacts. The project aims to develop new approaches inspired by our immune system to rapidly isolate pathogens from clinical samples without the need for culture. Such approaches will enable the use of advanced characterisation and detection assays (e.g. Next Generation Sequencing) and shorten time to informed diagnostics.
Job title: Engineering new proteins to detect pathogens in blood
Reporting To: Dr Melanie Jimenez
Department: University of Glasgow, Biomedical Engineering Division
Duration: 12 weeks
Placement Ref: EQU/2019/049
- Expressing and purifying target proteins for pathogen separation
- Characterising the potential of expressed proteins to separate pathogens by coating them onto magnetic particles
- Analysing the separation efficiency with model environment (e.g. PBS spiked with bacteria)
Knowledge and skills required:
– 3rd– 4th year of Undergraduate degree with Honours in Life Sciences; Biology, Biochemistry, Microbiology, Molecular Biology, Genetics, Biomedical Sciences or similar
– Experience related to laboratory skills in molecular biology and biochemistry (e.g. molecular cloning, protein expression using mammalian cell expression systems, protein purification, biochemical characterisation).
- Interest in interdisciplinary research, ability to work creatively as part of a small team. Passion for research.
- The successful applicant will work closely with a PhD student in the Institute of Infection, Immunity & Inflammation at the University of Glasgow.
|Education/Qualifications||3rd-4th year of Undergraduate degree with Honours in Life Sciences; Biology, Biochemistry, Microbiology, Molecular Biology, Genetics, Biomedical Sciences or similar||Completed Honours project|
|Experience||One substantial individual research lab placement (3+ months)||Additional projects / internships / interests in interdisciplinary research areas|
|Skills/Personal Requirements||Passion for research topic, comfortable working independently in a biological research laboratory and as part of a small team.|
Ability to reliably record results and design experiments, desire to pick up new skills
|Experience working with mammalian cell expression systems, tissue culture, chromatography-based protein purification, methods of biochemical characterisation|